MOLECULAR METHODOLOGIES APPLIED TO VEGETABLE SYSTEMS
Academic Year 2023/2024 - Teacher: GIUSEPPE PUGLIAExpected Learning Outcomes
The lecture aims to provide theoretical knowledge on the main molecular methodologies applied to Plant Biology. Particular emphasis is given to the in-depth study of the molecular mechanisms that regulate the response of plants to different environmental conditions. Laboratory activities are planned to provide students with hands-on experience with the main techniques of biomolecular analysis applied to plant organisms
Required Prerequisites
It is recommended to have passed the Plant Morphology and Physiology and Molecular Biology exams.
Attendance of Lessons
Course attendance is strongly recommended
Detailed Course Content
Organization, structure and characteristics of plant genomes: size, repeated elements and variability.
Sequencing methodologies of plant genomes and transcriptomes.
Techniques for analyzing genetic diversity in plant systems: intra- and inter-specific genetic variability (DNA barcoding)
The plant gene, role of promoter, terminator and transcriptional factors
Methods of genetic transformation in plants: direct or by genetic transformation vectors
Basic knowledge of specialized metabolism in plants.
Regulation of gene expression: transcriptional factors and epigenetics
Transduction of environmental signals and the role of phytohormones
Plant responses to abiotic stresses
Textbook Information
Biologia delle piante. Smith, Coupland, Dolan, Harberd, Jones, Martin, Sablowski & Amey, ultima edizione, Zanichelli editor
Learning Assessment
Learning Assessment Procedures
Assessment of learning consists of an oral examination.
Examples of frequently asked questions and / or exercises
What is the role of specialized metabolism in plants?
VERSIONE IN ITALIANO
What are the major regulatory systems of gene expression in plants?