Biochemical analysis for medical diagnostics

Type of biochemical-clinical analyzes. Single analysis, analysis groupings, biochemical profiles, function tests, screening tests, emergency exams.
Importance of patient preparation before collection. Sample collection procedure. Sample treatment and storage. Accuracy, accuracy and specificity of the methods used. Quality control. Reference values.
Fundamental techniques employed in the biochemical-clinical laboratory. Spectrophotometric, fluorimetric techniques. Electrophoresis, electrochemical techniques, immunological techniques, radioimmunoassays, enzymatic techniques.
Automatic systems for measuring the various biochemical parameters. Enzymes and enzymatic diagnostics. Considerations on the methods of measurement of enzymatic activities.
General characteristics of the most important enzymes of clinical interest. Isoenzymes: characteristics, distribution and their clinical significance. Changes in enzymatic activities in pathological conditions.
Plasma proteins. General features and functions of some proteins: chemical analysis methods. Electrophoresis.
Plasma lipoproteins. Cholesterol. Triglycerides. General characteristics of hormones. Changes in pathological conditions. Methods of determination of the main hormones.
Quantitative analysis of blood cells: counting of erythrocytes, counting of leukocytes and platelets. Hematocrit, hemoglobin. Classification of anemias.
Bilirubin in the blood. Alterations of bilirubin metabolism. The itteri.
VES. TAS. Blood groups. Coagulation and fibrinolysis.
Glucose metabolism. Blood glucose regulation and methods of determination. Diabetes. Load curves, post-prandial glycemia. Chetonic bodies.
Non-protein nitrogen. Azotemia and its meaning.
Creatine and creatinine. Uric acid and gout.
 

Type of biochemical-clinical analyzes. Single analysis, analysis groupings, biochemical profiles, function tests, screening tests, emergency exams.
Importance of patient preparation before collection. Sample collection procedure. Sample treatment and storage. Accuracy, accuracy and specificity of the methods used. Quality control. Reference values.
Fundamental techniques employed in the biochemical-clinical laboratory. Spectrophotometric, fluorimetric techniques. Electrophoresis, electrochemical techniques, immunological techniques, radioimmunoassays, enzymatic techniques.
Automatic systems for measuring the various biochemical parameters. Enzymes and enzymatic diagnostics. Considerations on the methods of measurement of enzymatic activities.
General characteristics of the most important enzymes of clinical interest. Isoenzymes: characteristics, distribution and their clinical significance. Changes in enzymatic activities in pathological conditions.
Plasma proteins. General features and functions of some proteins: chemical analysis methods. Electrophoresis.
Plasma lipoproteins. Cholesterol. Triglycerides. General characteristics of hormones. Changes in pathological conditions. Methods of determination of the main hormones.
Quantitative analysis of blood cells: counting of erythrocytes, counting of leukocytes and platelets. Hematocrit, hemoglobin. Classification of anemias.
Bilirubin in the blood. Alterations of bilirubin metabolism. The itteri.
VES. TAS. Blood groups. Coagulation and fibrinolysis.
Glucose metabolism. Blood glucose regulation and methods of determination. Diabetes. Load curves, post-prandial glycemia. Chetonic bodies.
Non-protein nitrogen. Azotemia and its meaning.
Creatine and creatinine. Uric acid and gout.