PATOLOGIA MOLECOLARE E DIAGNOSTICA DI LABORATORIO AVANZATO

The objectives of the Course are to provide the student with the molecular basis of oncological and autoimmune diseases and the knowledge on modern diagnostic techniques of the biomedical laboratory

Autoimmunity: molecular basis of autoimmunity, hypersensitivity reactions, nosographic classification of the main autoimmune diseases

Immunofluorescence: principles and applications in the diagnosis of autoimmune diseases

Transplant immunology: molecular basis and study techniques

Molecular basis of neoplasms: alteration of the mechanisms for maintaining genomic integrity; oncogenes and tumor suppressors

DNA methylation study: study of the MLH1 gene and the MGMT gene

Flow cytometry: principles and applications in the biomedical field and in the diagnostics of oncological diseases of the blood

Microarrays: principles, platforms, applications in the biomedical field

First and Next generation Sequencing: Sanger sequencing; Maxam-Gilbert sequencing; pyrosequencing; Illumina sequencing; nanopore sequencing

Applications of molecular pathology techniques to oncology: microsatellite instability, BRCA1-2 mutations, BRAFV600E mutations

Autoimmunity: molecular basis of autoimmunity, hypersensitivity reactions, nosographic classification of the main autoimmune diseases

Immunofluorescence: principles and applications in the diagnosis of autoimmune diseases

Transplant immunology: molecular basis and study techniques

Molecular basis of neoplasms: alteration of the mechanisms for maintaining genomic integrity; oncogenes and tumor suppressors

DNA methylation study: study of the MLH1 gene and the MGMT gene

Flow cytometry: principles and applications in the biomedical field and in the diagnostics of oncological diseases of the blood

First and Next generation Sequencing: Sanger sequencing; Maxam-Gilbert sequencing; pyrosequencing; Illumina sequencing; nanopore sequencing

Applications of molecular pathology techniques to oncology: microsatellite instability, BRCA1-2 mutations, BRAFV600E mutations

1. Abbas, Lichtman, Pillai. Immunologia cellulare e molecolare. Edra; 9° edizione 2018

2. McPherson. HENRY'S Diagnosi Clinica e Metodi di Laboratorio. Antonio Delfino Editore

3. Leonard. Molecular Pathology in Clinical Practice. Springer

5. Weinberg: La biologia del cancro. Zanichelli

6. Mauro Maccarrone. Metodologie Biochimiche e Biomolecolari. Zanichelli. 2019