GENETICA MOLECOLARE E BIOTECNOLOGIE MICROBICHEModule BIOTECNOLOGIE MICROBICHE
Academic Year 2023/2024 - Teacher: Maria Carmela SANTAGATIExpected Learning Outcomes
The aim of the Microbial Biotechnology module is to deepen the knowledge of microbial genomics addressing gene organization (whole genome sequencing,use of genomic databases and bioinformatics tools for data analysis), transcriptomics, mobile genetic elements and their mechanisms of gene transfer and recombinant DNA techniques focusing on their application in the biotechnological field. During the course knowledge on the microbiota/human microbiome with metagenomic analyses and on bacterial strains producing bacteriocins with probiotic characteristics isdeepened. Following the course students also develop an integrated understanding of biological phenomena and advanced scientific preparation at the morphological/functional, cellular/molecular level of microorganisms
Course Structure
Required Prerequisites
Detailed Course Content
Microbial genomes and molecular genetics of bacteria. Studies of microbial genomes and characterization of mobile elements such as transposons and plasmids. Principles of DNA sequencing, techniques of mutagenesis, PCR, design primers and probes, Real-Time PCR for the study of bacterial genetics. Sequence analysis and use of bioinformatics tools. Study of CRISPR systems (Clustered Regularly interspaced Short palindromic Repeats) and its applications. Application of molecular diagnostics (DNA and immunology, monoclonal antibodies). Probiotics, bacteriotherapy and their applications, microbiomi analysis. Study of toxin and antitoxin systems (TA), sRNA and riboswitch.
Textbook Information
- Biologia dei microrganismi, Gianni Deho, Enrica Galli
- Molecular Genetics of Bacteria- Jeremy Dale Editor John Wiley and Sons
- scientific publications